Serotonin-mediated modulation of Na+/K+ pump current in rat hippocampal CA1 pyramidal neurons

BMC Neurosci. 2012 Jan 19;13:10. doi: 10.1186/1471-2202-13-10.

Abstract

Background: The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na+/K+ pump in rat hippocampal CA1 pyramidal neurons.

Results: 5-HT (0.1, 1 mM) showed Na+/K+ pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT3 receptor (5-HT3R) antagonist, not by WAY100635, a 5-HT1AR antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT3R specific agonist, mimicked the effect of 5-HT on Ip.

Conclusion: 5-HT inhibits neuronal Na+/K+ pump activity via 5-HT3R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Biguanides / pharmacology
  • Biophysics
  • CA1 Region, Hippocampal / cytology*
  • Dose-Response Relationship, Drug
  • Electric Stimulation
  • In Vitro Techniques
  • Neural Inhibition / drug effects
  • Patch-Clamp Techniques
  • Piperazines / pharmacology
  • Pyramidal Cells / drug effects*
  • Pyramidal Cells / physiology
  • Pyridines / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin / pharmacology*
  • Serotonin Antagonists / pharmacology
  • Serotonin Receptor Agonists / pharmacology
  • Sodium Channel Blockers / pharmacology
  • Sodium-Potassium-Exchanging ATPase / metabolism*
  • Strophanthidin / pharmacology
  • Tetrodotoxin / pharmacology

Substances

  • Biguanides
  • Piperazines
  • Pyridines
  • Serotonin Antagonists
  • Serotonin Receptor Agonists
  • Sodium Channel Blockers
  • Serotonin
  • Tetrodotoxin
  • Strophanthidin
  • N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide
  • 1-(3-chlorophenyl)biguanide
  • Sodium-Potassium-Exchanging ATPase