Phenotypic, biochemical, and molecular techniques for detection of metallo-β-lactamase NDM in Acinetobacter baumannii

J Clin Microbiol. 2012 Apr;50(4):1419-21. doi: 10.1128/JCM.06276-11. Epub 2012 Jan 18.

Abstract

Seven NDM-positive Acinetobacter baumannii isolates of worldwide origin were studied to evaluate the best technique for their identification. Detection of carbapenemase producers based on the measurement of carbapenemase activity by UV spectrophotometry (as for A. baumannii strains producing other types of carbapenemase), or by the modified Hodge test, failed. Inhibition activity using EDTA was a sensitive technique but lacked specificity compared to molecular techniques, which remain the gold standard.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / drug effects
  • Acinetobacter baumannii / enzymology*
  • Acinetobacter baumannii / genetics
  • Disk Diffusion Antimicrobial Tests
  • Molecular Typing
  • Phenotype*
  • Polymerase Chain Reaction
  • beta-Lactam Resistance
  • beta-Lactamases / chemistry
  • beta-Lactamases / genetics*
  • beta-Lactams / pharmacology*

Substances

  • beta-Lactams
  • beta-Lactamases
  • beta-lactamase NDM-1