Magnesium absorption from mineral water decreases with increasing quantities of magnesium per serving in rats

Nutr Res. 2012 Jan;32(1):59-65. doi: 10.1016/j.nutres.2011.11.001. Epub 2011 Dec 29.


It is hypothesized that magnesium (Mg) absorption from mineral water is affected by the concentration of Mg in the water, the consumption pattern, and the volume consumed per serving. The present study examined the effect of serving volume and consumption pattern of artificial mineral water (AMW) and Mg concentration on Mg absorption in rats. Magnesium in AMW was labeled with magnesium-25 as a tracer. Each group consisted of 6 or 7 rats. In experiment 1, the rats received 1 mL of AMW containing 200 mg Mg/L at 4 times, 400 mg Mg/L twice, or 800 mg Mg/L at 1 time. In experiment 2, the rats received 1 mL of AMW containing 200 mg Mg/L or 0.25 mL of AMW containing 800 mg Mg/L at 4 times or 1 mL of AMW containing 800 mg Mg/L at 1 time. The absorption of Mg decreased with increasing Mg concentrations in the same serving volume of AMW with different serving frequencies. When the AMW containing 800 mg Mg/L was portioned into 4 servings, Mg absorption increased to the level of absorption in the group exposed to AMW containing 200 mg Mg/L served at the same frequency. These results suggest that the Mg concentration and the volume of AMW do not affect Mg absorption per se, but Mg absorption from AMW decreases when the amount of Mg in each serving is increased. Thus, frequent consumption is preferable for mineral water rich in Mg when the total consumption of mineral water is the same.

MeSH terms

  • Absorption
  • Animals
  • Diet
  • Drinking Water / administration & dosage*
  • Magnesium / pharmacokinetics*
  • Male
  • Mineral Waters / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley


  • Drinking Water
  • Mineral Waters
  • Magnesium