Mucolysis by Ascorbic Acid and Hydrogen Peroxide on Compact Mucin Secreted in Pseudomyxoma Peritonei

J Surg Res. 2012 May 15;174(2):e69-73. doi: 10.1016/j.jss.2011.10.038. Epub 2011 Nov 19.

Abstract

Background: This study examines the potential efficacy of hydrogen peroxide and ascorbic acid in the dissolution of mucinous ascites from pseudomyxoma peritonei.

Methods: The mucolytic action of both ascorbic acid (0%-0.2%) and hydrogen peroxide (0%-3%) are investigated as single agent on mucin samples derived from patient. This was followed by examining the joint action of ascorbic acid (0.2%) and hydrogen peroxide (0%-3.0%) on mucin. To lower the concentration of hydrogen peroxide in the mixture, the action of equal concentration of ascorbic acid/hydrogen peroxide ranging from 0%-0.3% are then examined. Finally, the pH (4.5-7.0) effect on mucolytic properties of equal concentration (0.2%) of ascorbic acid/hydrogen peroxide was studied.

Results: At the concentrations examined (0%-0.2%), ascorbic acid showed highest mucolytic activity at 0.2%. Similarly, hydrogen peroxide as a single agent (0%-3.0%) showed highest mucolytic activity at 3.0%. The mucolytic action of hydrogen peroxide (0%-3.0%) containing 0.2% ascorbic acid demonstrated synergistic effects. At equal concentration of the two agents, ranging from 0%-0.5%, maximal mucolytic action was observed at 0.2%. The mucolytic property of the final mixture (0.2% ascorbic acid/0.2% hydrogen peroxide) was pH-dependent and showed maximal degradation at pH 4.5 and declined as it reached towards neutral pH.

Conclusion: The current study introduces the potential applicability of a formulation that holds promise as a mucolytic agent in patients with mucinous ascites from pseudomyxoma peritonei.

MeSH terms

  • Ascorbic Acid / pharmacology
  • Ascorbic Acid / therapeutic use*
  • Drug Evaluation, Preclinical
  • Expectorants / pharmacology
  • Expectorants / therapeutic use*
  • Humans
  • Hydrogen Peroxide / pharmacology
  • Hydrogen Peroxide / therapeutic use*
  • Mucins / drug effects
  • Peritoneal Neoplasms / drug therapy*
  • Pseudomyxoma Peritonei / drug therapy*

Substances

  • Expectorants
  • Mucins
  • Hydrogen Peroxide
  • Ascorbic Acid