Inhalation of hydrogen gas protects against myocardial stunning and infarction in swine

Scand Cardiovasc J. 2012 Jun;46(3):183-9. doi: 10.3109/14017431.2012.659676. Epub 2012 Feb 20.

Abstract

Objectives: The present study was carried out to determine whether inhalation of hydrogen (H(2)) gas protects myocardium against ischemia-reperfusion (I/R) injury in swine.

Design: In anesthetized open-chest swine, myocardial stunning was produced by 12-minute occlusion of left anterior descending coronary artery (LAD) followed by 90-minute reperfusion in the first study. Group A inhaled 100% oxygen, and group B inhaled 2% H(2) plus 98% oxygen during ischemia and reperfusion. In the second study, myocardial infarction was produced by 40-minute occlusion of LAD followed by 120-minute reperfusion. Group C inhaled 100% oxygen during ischemia and reperfusion. Group D inhaled 2% H(2) plus 98% oxygen. Group E inhaled 4% H(2) plus 96% oxygen.

Results: The change of segment shortening (%SS) from baseline at 90 minutes after reperfusion in group B was 74 ± 13 (mean ± SD) %, which was significantly higher than that in group A (48 ± 15%). Myocardial infarct size in group E (32 ± 10%), but not in group D (40 ± 9%) was smaller than that in group C (46 ± 6%).

Conclusions: Inhalation of 2% H(2) gas improves myocardial stunning, and inhalation of 4% but not 2% H(2) gas reduces myocardial infarct size in swine.

MeSH terms

  • Administration, Inhalation
  • Animals
  • Cardiotonic Agents / administration & dosage*
  • Disease Models, Animal
  • Female
  • Gases
  • Hydrogen / administration & dosage*
  • Male
  • Myocardial Contraction / drug effects
  • Myocardial Infarction / pathology
  • Myocardial Infarction / physiopathology
  • Myocardial Infarction / prevention & control*
  • Myocardial Reperfusion Injury / pathology
  • Myocardial Reperfusion Injury / physiopathology
  • Myocardial Reperfusion Injury / prevention & control*
  • Myocardial Stunning / pathology
  • Myocardial Stunning / physiopathology
  • Myocardial Stunning / prevention & control*
  • Myocardium / pathology
  • Swine
  • Time Factors
  • Ventricular Function, Left / drug effects
  • Ventricular Pressure / drug effects

Substances

  • Cardiotonic Agents
  • Gases
  • Hydrogen