HPLC-DAD-MS/MS profiling of antioxidant flavonoid glycosides in sea buckthorn (Hippophae rhamnoides L.) seeds

Int J Food Sci Nutr. 2012 Sep;63(6):730-8. doi: 10.3109/09637486.2011.652075. Epub 2012 Jan 20.


This study was aimed at the chemical profiling of flavonoid glycosides in antioxidant (AO) fractions of sea buckthorn (Hippophae rhamnoides) seed. Seed fractions were evaluated for their DPPH, ABTS, superoxide and hydroxyl radical scavenging, ferric reduction, ferrous chelation and xanthine oxidase inhibitory capacities. HPLC-DAD-ESI-MS/MS analytical conditions for the profiling of seed flavonoids were optimized and the AO-rich fraction was analysed. Quercetin-3-O-rutinoside (5.9%), isorhamnetin-3-O-rutinoside (4.9%) and isorhamnetin-3-O-sophroside-7-O-rhamnoside (3.7%) were found as the major flavonoid glycosides in the fraction. Significant amounts of isorhamnetin-3-O-glucoside (2.8%), 3-O-sophroside-7-O-rhamnosides of quercetin (2.4%) and kaempherol (1.3%), and 3-O-glucoside-7-O-rhamnosides of quercetin (1.1%) and isorhamnetin (1.1%) along with their free forms: isorhamnetin (2.7%), quercetin (1.1%) and kaempherol (0.6%) were also found in the fraction. The identification of flavonoids as the major less polar AO phenolics in the seeds was rationalized by demonstrating the high AO activity of isorhamnetin, quercetin, kaempherol and quercetin-3-O-rutinoside.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Flavonoids / chemistry*
  • Glycosides / chemistry*
  • Hippophae / chemistry*
  • Hydroxyl Radical
  • Iron Chelating Agents / chemistry
  • Molecular Structure
  • Seeds / chemistry*
  • Tandem Mass Spectrometry / methods*
  • Xanthine Oxidase / antagonists & inhibitors


  • Antioxidants
  • Flavonoids
  • Glycosides
  • Iron Chelating Agents
  • Hydroxyl Radical
  • Xanthine Oxidase