Effect of TGF-beta 1, TGF-beta 2, and bFGF on chick cartilage and muscle cell differentiation

Exp Cell Res. 1990 Nov;191(1):144-8. doi: 10.1016/0014-4827(90)90048-f.

Abstract

In insulin containing defined medium TGF-beta 1, TGF-beta 2, and bFGF all stimulate chondrogenic differentiation in high-density micromass cultures of distal limb bud mesenchyme cells of chick embryos. In addition bFGF inhibits myogenic differentiation, while TGF-beta 1 and TGF-beta 2 appear to have no effect. TGF-beta 1 and bFGF together act additively to enhance chondrogenesis, while TGF-beta blocks the bFGF inhibitory action on myoblasts, thus allowing them to differentiate. In the absence of insulin, the inhibitory effect of bFGF on muscle cell differentiation is reduced; cartilage differentiation in the presence of the above growth factors is also slightly reduced.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cartilage / cytology
  • Cartilage / drug effects*
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Chick Embryo
  • Fibroblast Growth Factor 2 / pharmacology*
  • Immunoenzyme Techniques
  • Muscles / cytology
  • Muscles / drug effects*
  • Myosins / metabolism
  • Transforming Growth Factor beta / pharmacology*

Substances

  • Antibodies, Monoclonal
  • Transforming Growth Factor beta
  • Fibroblast Growth Factor 2
  • Myosins