Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 30 (2), 174-8

Genome Sequencing Reveals Agronomically Important Loci in Rice Using MutMap


Genome Sequencing Reveals Agronomically Important Loci in Rice Using MutMap

Akira Abe et al. Nat Biotechnol.


The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type line and then selfed, allowing unequivocal segregation in second filial generation (F(2)) progeny of subtle phenotypic differences. This approach is particularly amenable to crop species because it minimizes the number of genetic crosses (n = 1 or 0) and mutant F(2) progeny that are required. We applied MutMap to seven mutants of a Japanese elite rice cultivar and identified the unique genomic positions most probable to harbor mutations causing pale green leaves and semidwarfism, an agronomically relevant trait. These results show that MutMap can accelerate the genetic improvement of rice and other crop plants.

Similar articles

See all similar articles

Cited by 241 PubMed Central articles

See all "Cited by" articles


    1. Plant J. 2006 Sep;47(6):969-76 - PubMed
    1. Nature. 2002 Apr 18;416(6882):701-2 - PubMed
    1. Mutat Res. 1993 Jul;288(1):123-31 - PubMed
    1. Genome Res. 2008 Nov;18(11):1851-8 - PubMed
    1. J Biosci Bioeng. 2007 Jul;104(1):34-41 - PubMed

Publication types

LinkOut - more resources