Biosynthesis of cyclic 2,3-diphosphoglycerate. Isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from Methanothermus fervidus

FEBS Lett. 1990 Oct 15;272(1-2):94-8. doi: 10.1016/0014-5793(90)80456-s.

Abstract

Starting from 2-phosphoglycerate the biosynthesis of cDPG comprises two steps: (i) the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate and (ii) the intramolecular cyclization to cyclic 2,3-diphosphoglycerate. The involved enzymes, 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase, were purified form Methanothermus fervidus. Their molecular and catalytic properties were characterized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,3-Diphosphoglycerate*
  • Adenosine Triphosphate / metabolism
  • Archaeal Proteins*
  • Diphosphoglyceric Acids / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Euryarchaeota / enzymology*
  • Glyceric Acids / metabolism
  • Kinetics
  • Lyases / chemistry
  • Lyases / isolation & purification*
  • Lyases / metabolism
  • Macromolecular Substances
  • Molecular Weight
  • Phosphoglycerate Kinase / chemistry
  • Phosphoglycerate Kinase / isolation & purification*
  • Phosphoglycerate Kinase / metabolism
  • Phosphorus-Oxygen Lyases*
  • Potassium / pharmacology

Substances

  • Archaeal Proteins
  • Diphosphoglyceric Acids
  • Glyceric Acids
  • Macromolecular Substances
  • 2,3-Diphosphoglycerate
  • 2-phosphoglycerate
  • cyclic-2,3-diphosphoglycerate
  • Adenosine Triphosphate
  • Phosphoglycerate Kinase
  • Lyases
  • Phosphorus-Oxygen Lyases
  • cdpgs protein, Methanothermus fervidus
  • Potassium