Nisin F-loaded brushite bone cement prevented the growth of Staphylococcus aureus in vivo

J Appl Microbiol. 2012 Apr;112(4):831-40. doi: 10.1111/j.1365-2672.2012.05241.x. Epub 2012 Feb 13.


Aims: To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo.

Methods and results: Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w) and the cement moulded into cylinders. In vitro antibacterial activity was determined using a delayed agar diffusion assay. Release of nisin F from the cement was determined using BCA protein assays. Based on scanning electron microscopy and X-ray diffraction analysis, nisin F did not cause significant changes in cement structure or chemistry. Cement containing 5·0% (w/w) nisin F yielded the most promising in vitro results. Nisin F-loaded cement was implanted into a subcutaneous pocket on the back of mice and then infected with S. aureus Xen 36. Infection was monitored for 7 days, using an in vivo imaging system. Nisin F prevented S. aureus infection for 7 days and no viable cells were isolated from the implants.

Conclusions: Nisin F-loaded brushite cement successfully prevented in vivo growth of S. aureus.

Significance and impact of the study: Nisin F incorporated into bone cement may be used to control S. aureus infection in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bone Cements / chemistry*
  • Calcium Phosphates / chemistry*
  • Mice
  • Microscopy, Electron, Scanning
  • Nisin / analogs & derivatives*
  • Nisin / pharmacology
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / growth & development*
  • X-Ray Diffraction


  • Anti-Bacterial Agents
  • Bone Cements
  • Calcium Phosphates
  • nisin F, Lactococcus lactis
  • Nisin
  • calcium phosphate, dibasic, dihydrate