Unusual NADPH conformation in the crystal structure of a cinnamyl alcohol dehydrogenase from Helicobacter pylori in complex with NADP(H) and substrate docking analysis

FEBS Lett. 2012 Feb 17;586(4):337-43. doi: 10.1016/j.febslet.2012.01.020. Epub 2012 Jan 21.


Cinnamyl alcohol dehydrogenase is a zinc- and NADPH-dependent dehydrogenase catalyzing the reversible conversion of p-hydroxycinnamaldehydes to their corresponding hydroxycinnamyl alcohols. A CAD homolog from Helicobacter pylori (HpCAD) possesses broad substrate specificities like the plant CADs and additionally a dismutation activity converting benzaldehyde to benzyl alcohol and benzoic acid. We have determined the crystal structure of HpCAD complexed with NADP(H) at 2.18Å resolution to get a better understanding of this class of CAD outside of plants. The structure of HpCAD is highly homologous to the sinapyl alcohol dehydrogenase and the plant CAD with well-conserved residues involved in catalysis and zinc binding. However, the NADP(H) binding mode of the HpCAD has been found to be significantly different from those of plant CADs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / chemistry*
  • Alcohol Oxidoreductases / genetics
  • Alcohol Oxidoreductases / metabolism
  • Amino Acid Substitution
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Catalytic Domain
  • Crystallography, X-Ray
  • DNA, Bacterial / genetics
  • Dimerization
  • Helicobacter pylori / enzymology*
  • Helicobacter pylori / genetics
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • NAD / chemistry
  • NADP / chemistry*
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism
  • Protein Conformation
  • Protein Structure, Quaternary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Substrate Specificity


  • Bacterial Proteins
  • DNA, Bacterial
  • Plant Proteins
  • Recombinant Proteins
  • NAD
  • NADP
  • Alcohol Oxidoreductases
  • cinnamyl alcohol dehydrogenase

Associated data

  • PDB/3WTO