Microsatellite marker development for the threatened orchid Masdevallia solomonii (Orchidaceae)

Am J Bot. 2012 Feb;99(2):e66-8. doi: 10.3732/ajb.1100364. Epub 2012 Jan 24.

Abstract

Premise of the study: Microsatellite markers for Masdevallia solomonii were developed to serve as a tool in future population genetic studies of this threatened species from the Bolivian Yungas.

Methods and results: Thirteen microsatellite primers were characterized by cloning an intersimple sequence repeat (ISSR) library. From these, 10 loci presented considerable variation in allele number (3-10), expected heterozygosity (0.537-0.865), and polymorphic information content per locus (0.500-0.848).

Conclusions: The markers obtained for M. solomonii are the first in the genus and subtribe. The observed polymorphism will make it possible to assess genetic diversity and structure of this species and will serve to propose effective conservation actions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Plant / genetics
  • Endangered Species*
  • Gene Frequency
  • Gene Library
  • Genetic Loci
  • Genotyping Techniques
  • Heterozygote
  • Microsatellite Repeats*
  • Orchidaceae / genetics*
  • Polymorphism, Genetic

Substances

  • DNA Primers
  • DNA, Plant