Asymmetric segregation of polarized antigen on B cell division shapes presentation capacity

Science. 2012 Jan 27;335(6067):475-9. doi: 10.1126/science.1214100.

Abstract

During the activation of humoral immune responses, B cells acquire antigen for subsequent presentation to cognate T cells. Here we show that after mouse B cells accumulate antigen, it is maintained in a polarized distribution for extended periods in vivo. Using high-throughput imaging flow cytometry, we observed that this polarization is preserved during B cell division, promoting asymmetric antigen segregation among progeny. Antigen inheritance correlates with the ability of progeny to activate T cells: Daughter cells receiving larger antigen stores exhibit a prolonged capacity to present antigen, which renders them more effective in competing for T cell help. The generation of progeny with differential capacities for antigen presentation may have implications for somatic hypermutation and class switching during affinity maturation and as B cells commit to effector cell fates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation*
  • Antigens / analysis*
  • Antigens / immunology*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Cell Division
  • Cell Proliferation
  • Cells, Cultured
  • Coculture Techniques
  • Computer Simulation
  • Flow Cytometry
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Immunological
  • Muramidase / analysis
  • Muramidase / immunology
  • T-Lymphocytes / immunology*

Substances

  • Antigens
  • hen egg lysozyme
  • Muramidase