Evidence for the role of 34-kDa galactoside-binding lectin in transformation and metastasis

Int J Cancer. 1990 Nov 15;46(5):871-7. doi: 10.1002/ijc.2910460520.


The endogenous Mr 34,000 galactoside-binding lectin (L-34) is found at elevated levels in a wide variety of neoplastic cells and correlative evidence suggests that it is involved in tumor metastasis in vivo and in transformation in vitro. We demonstrate here that introduction of recombinant L-34 into tumorigenic, weakly metastatic UV-2237-cl-15 fibrosarcoma cells results in an increased incidence of experimental lung metastases in syngeneic and nude mice. Transfection of normal BALB/c-A31 cloned fibroblasts with functional L-34 results in acquisition of anchorage-independent growth and in morphological transformation in vitro but not in tumorigenicity in vivo. These results provide direct evidence that the cellular expression of L-34 is associated with some aspects of transformation and with metastasis, but not with tumorigenicity per se.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Division / physiology
  • Cell Transformation, Neoplastic / pathology*
  • DNA / genetics
  • Fibroblasts / cytology
  • Fibroblasts / physiology
  • Fibrosarcoma / genetics
  • Fibrosarcoma / pathology
  • Galectins
  • Hemagglutinins / genetics
  • Hemagglutinins / pharmacology
  • Hemagglutinins / physiology*
  • Mice
  • Neoplasm Metastasis / genetics
  • Neoplasm Metastasis / physiopathology*
  • Recombinant Proteins / genetics
  • Transfection
  • Tumor Cells, Cultured


  • Galectins
  • Hemagglutinins
  • Recombinant Proteins
  • DNA