Rolling circle amplification-templated DNA nanotubes show increased stability and cell penetration ability

J Am Chem Soc. 2012 Feb 15;134(6):2888-91. doi: 10.1021/ja2107492. Epub 2012 Feb 2.


DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length. These nanotubes are more resistant to nuclease degradation, capable of entering human cervical cancer (HeLa) cells with significantly increased uptake over double-stranded DNA, and are amenable to encapsulation and release behavior. As such, they represent a potentially unique platform for the development of cell probes, drug delivery, and imaging tools.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • DNA Primers
  • DNA-Directed DNA Polymerase / genetics
  • Deoxyribonucleases / metabolism
  • Drug Delivery Systems
  • Gene Amplification*
  • HeLa Cells
  • Humans
  • Mutation
  • Nanocomposites / chemistry
  • Nanotubes / chemistry*
  • Nucleic Acid Amplification Techniques
  • RNA Probes
  • Templates, Genetic


  • DNA Primers
  • RNA Probes
  • DNA
  • DNA-Directed DNA Polymerase
  • Deoxyribonucleases