Cloning and sequencing of inulinase and β-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes

Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27.

Abstract

An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.

MeSH terms

  • Alteromonadaceae / classification
  • Alteromonadaceae / enzymology*
  • Alteromonadaceae / genetics
  • Alteromonadaceae / isolation & purification
  • Cloning, Molecular*
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / isolation & purification
  • Glycoside Hydrolases / metabolism
  • Inulin / metabolism
  • Molecular Sequence Data
  • Open Reading Frames
  • Operon
  • RNA, Ribosomal, 16S / genetics
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Substrate Specificity
  • beta-Fructofuranosidase / chemistry
  • beta-Fructofuranosidase / genetics*
  • beta-Fructofuranosidase / isolation & purification
  • beta-Fructofuranosidase / metabolism

Substances

  • RNA, Ribosomal, 16S
  • Recombinant Proteins
  • Inulin
  • Glycoside Hydrolases
  • beta-Fructofuranosidase
  • inulinase

Associated data

  • GENBANK/AB669003
  • GENBANK/AB669413
  • GENBANK/AB669414
  • GENBANK/AB669415