Abstract
An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.
MeSH terms
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Alteromonadaceae / classification
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Alteromonadaceae / enzymology*
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Alteromonadaceae / genetics
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Alteromonadaceae / isolation & purification
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Cloning, Molecular*
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Glycoside Hydrolases / chemistry
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / isolation & purification
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Glycoside Hydrolases / metabolism
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Inulin / metabolism
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Molecular Sequence Data
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Open Reading Frames
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Operon
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RNA, Ribosomal, 16S / genetics
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism*
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Seawater / microbiology*
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Sequence Analysis, DNA
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Substrate Specificity
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beta-Fructofuranosidase / chemistry
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beta-Fructofuranosidase / genetics*
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beta-Fructofuranosidase / isolation & purification
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beta-Fructofuranosidase / metabolism
Substances
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RNA, Ribosomal, 16S
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Recombinant Proteins
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Inulin
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Glycoside Hydrolases
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beta-Fructofuranosidase
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inulinase
Associated data
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GENBANK/AB669003
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GENBANK/AB669413
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GENBANK/AB669414
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GENBANK/AB669415