Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells

FEBS Lett. 2012 Feb 17;586(4):442-7. doi: 10.1016/j.febslet.2012.01.027. Epub 2012 Jan 28.


miRNAs are a class of small-noncoding RNAs capable of negatively regulating gene expression. Here, we found that miR-195 is down-regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role of miR-195 in bladder cancer, we conducted gain of function analysis by transfecting bladder cancer cell line T24 with chemically synthesized miR-195 mimic. We identified CDK4, an early G1 cell cycle regulator, as a novel target of miR-195. Selective over-expression of miR-195 could induce G1-phase arrest in T24 cells, and subsequently inhibit T24 cell growth. These findings indicate that miR-195 could be a potential tumor suppressor in bladder cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Cycle Checkpoints / genetics*
  • Cell Cycle Checkpoints / physiology*
  • Cell Line, Tumor
  • Cell Proliferation
  • Cyclin-Dependent Kinase 4 / genetics*
  • Cyclin-Dependent Kinase 4 / metabolism*
  • Down-Regulation
  • G1 Phase
  • Humans
  • MicroRNAs / genetics*
  • RNA, Neoplasm / genetics
  • Tumor Stem Cell Assay
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / metabolism*
  • Urinary Bladder Neoplasms / pathology


  • MIRN195 microRNA, human
  • MicroRNAs
  • RNA, Neoplasm
  • CDK4 protein, human
  • Cyclin-Dependent Kinase 4