Recombinant human activin-A promotes proliferation of human luteinized preovulatory granulosa cells in vitro

J Clin Endocrinol Metab. 1990 Nov;71(5):1396-8. doi: 10.1210/jcem-71-5-1396.


Ovarian granulosa cells synthesize and secrete activin, a member of the transforming growth factor-beta (TGF-beta) peptide family, during the follicular phase of the menstrual cycle. We examined the growth-promoting activity of human recombinant activin-A on human luteinized preovulatory granulosa cells obtained from women undergoing in vitro fertilization. Activin-A induced proliferation of granulosa cells on day 5 of culture in a dose-dependent manner. Maximal effects were seen at concentrations greater than or equal to 100 ng/mL with an ED50 of 15 ng/mL. We suggest that activin-A is able to modulate growth of ovarian granulosa cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activins
  • Cell Division / drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Female
  • Fertilization in Vitro
  • Granulosa Cells / drug effects*
  • Humans
  • Inhibins / pharmacology*
  • Recombinant Proteins / pharmacology


  • Recombinant Proteins
  • Activins
  • Inhibins