Flow cytometry of fluorescent proteins

Methods. 2012 Jul;57(3):318-30. doi: 10.1016/j.ymeth.2012.01.003. Epub 2012 Jan 24.

Abstract

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.

Publication types

  • Review

MeSH terms

  • Bacterial Proteins / chemistry*
  • Cells, Cultured
  • Escherichia coli / genetics
  • Flow Cytometry / methods*
  • Fluorescent Dyes
  • Genetic Vectors
  • Green Fluorescent Proteins / chemistry*
  • Humans
  • Luminescent Proteins / chemistry*
  • Recombinant Fusion Proteins / chemistry
  • Spectrometry, Fluorescence

Substances

  • Bacterial Proteins
  • Fluorescent Dyes
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • fluorescent protein 583
  • red fluorescent protein
  • yellow fluorescent protein, Bacteria
  • Green Fluorescent Proteins