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, 7 (3), 512-6

Hypermethylation of p16 and DAPK Promoter Gene Regions in Patients With Non-Invasive Urinary Bladder Cancer


Hypermethylation of p16 and DAPK Promoter Gene Regions in Patients With Non-Invasive Urinary Bladder Cancer

Zbigniew Jabłonowski et al. Arch Med Sci.


Introduction: The aim of the study was to examine the frequency of methylation status in promoter regions of p16 and DAPK genes in patients with non-invasive bladder cancer.

Material and methods: Forty-two patients (92.9% men, 73.8% smokers, 71.4% T1G1, 19.1% T1G2, 9.5% T1G3) and 36 healthy controls were studied. Isolation of genomic DNA from blood serum and methylation-specific PCR (MSP) were applied. Methylation status - methylated and unmethylated promoter regions of p16 and DAPK genes were analysed.

Results: Seventeen out of 42 patients (40.5%) had the methylated p16 gene, while methylation of the DAPK gene was seen in 27 of 42 cases (64.3%). In 12 patients (28.6%) both analysed genes were methylated. A statistically significant (p = 0.046) higher frequency of DAPK gene methylation (71.4%) was observed in patients with lower grade (G1) bladder cancer.

Conclusions: Detection of the aberrant hypermethylation of DAPK and p16 genes in blood DNA from non-invasive bladder cancer patients might offer an effective means for earlier auxiliary diagnosis of the malignancy.

Keywords: DAPK; hypermethylation; methylation-specific PCR; non-invasive bladder cancer; p16.

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