Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 7 (3), 512-6

Hypermethylation of p16 and DAPK Promoter Gene Regions in Patients With Non-Invasive Urinary Bladder Cancer

Affiliations

Hypermethylation of p16 and DAPK Promoter Gene Regions in Patients With Non-Invasive Urinary Bladder Cancer

Zbigniew Jabłonowski et al. Arch Med Sci.

Abstract

Introduction: The aim of the study was to examine the frequency of methylation status in promoter regions of p16 and DAPK genes in patients with non-invasive bladder cancer.

Material and methods: Forty-two patients (92.9% men, 73.8% smokers, 71.4% T1G1, 19.1% T1G2, 9.5% T1G3) and 36 healthy controls were studied. Isolation of genomic DNA from blood serum and methylation-specific PCR (MSP) were applied. Methylation status - methylated and unmethylated promoter regions of p16 and DAPK genes were analysed.

Results: Seventeen out of 42 patients (40.5%) had the methylated p16 gene, while methylation of the DAPK gene was seen in 27 of 42 cases (64.3%). In 12 patients (28.6%) both analysed genes were methylated. A statistically significant (p = 0.046) higher frequency of DAPK gene methylation (71.4%) was observed in patients with lower grade (G1) bladder cancer.

Conclusions: Detection of the aberrant hypermethylation of DAPK and p16 genes in blood DNA from non-invasive bladder cancer patients might offer an effective means for earlier auxiliary diagnosis of the malignancy.

Keywords: DAPK; hypermethylation; methylation-specific PCR; non-invasive bladder cancer; p16.

Similar articles

See all similar articles

Cited by 20 PubMed Central articles

See all "Cited by" articles

References

    1. Bombail V, Moggs JG, Orphanides G. Perturbation of epigenetic status by toxicants. Toxicol Let. 2004;149:51–8. - PubMed
    1. Moggs JG, Goodman JI, Trosko JE, Roberts RA. Epigenetics and cancer: implications for drug discovery and safety assessment. Toxicol Appl Pharmacol. 2004;196:442–30. - PubMed
    1. Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. PNAS. 1996;93:9821–6. - PMC - PubMed
    1. Velentza AV, Schumacher AM, Weiss C, Egli M, Watterson DM. A protein kinase associted with apoptosis and tumor suppression. J Biol Chem. 2001;276:38956–65. - PubMed
    1. Yukawa K, Hoshino K, Kishino M, et al. Deletion of the kinase domain in death-associated protein kinase attenuates tubular cell apoptosis in chronic obstructive uropathy. Int J Mol Med. 2004;13:515–20. - PubMed

LinkOut - more resources

Feedback