Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis

Histopathology. 2012 Mar;60(4):561-9. doi: 10.1111/j.1365-2559.2011.04159.x. Epub 2012 Feb 1.


Aims: Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis.

Methods and results: mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers.

Conclusions: Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Appendicitis / genetics
  • Appendicitis / metabolism*
  • Appendicitis / pathology
  • Appendix / metabolism*
  • Appendix / pathology
  • Calcium-Binding Proteins
  • Child
  • DNA-Binding Proteins
  • Enterocytes / metabolism*
  • Enterocytes / pathology
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Humans
  • Male
  • Middle Aged
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Tumor Suppressor Proteins


  • Calcium-Binding Proteins
  • DMBT1 protein, human
  • DNA-Binding Proteins
  • Receptors, Cell Surface
  • Tumor Suppressor Proteins