Model system for cell adhesion mediated by weak carbohydrate-carbohydrate interactions

J Am Chem Soc. 2012 Feb 22;134(7):3326-9. doi: 10.1021/ja210304j. Epub 2012 Feb 10.


The multivalent carbohydrate-carbohydrate interaction between membrane-anchored epitopes derived from the marine sponge Microciona prolifera has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane-coated surfaces was employed to mimic native cell-cell contacts.The dynamic strength of the homomeric self-association was measured as a function of calcium ions and loading rate. A deterministic model was used to estimate the basic energy landscape and number of participating bonds in the contact zone.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Disaccharides / chemistry*
  • Disaccharides / isolation & purification
  • Lipid Bilayers / chemistry*
  • Microscopy, Scanning Probe
  • Porifera / chemistry*


  • Disaccharides
  • Lipid Bilayers