Efficacy of herbal essential oils as insecticide against Aedes aegypti (Linn.), Culex quinquefasciatus (Say) and Anopheles dirus (Peyton and Harrison)

Southeast Asian J Trop Med Public Health. 2011 Sep;42(5):1083-92.


The essential oils of Cananga odorata (ylang ylang), Citrus sinensis (orange), Cymbopogon citratus (lemongrass), Cymbopogon nardus (citronella grass), Eucalyptus citriodora (eucalyptus), Ocimum basilicum (sweet basil) and Syzygium aromaticum (clove), were tested for their insecticide activity against Aedes aegypti, Culex quinquefasciatus and Anopheles dirus using the WHO standard susceptibility test. These were applied in soybean oil at dose of 1%, 5% and 10% (w/v). C. citratus had the KT, values against the three mosquito species tested but the knockdown rates (at 10, 30 and 60 minutes) were lower than some essential oils. C. citratus oil had high insecticidal activity against Ae. aegypti, Cx. quinquefasciatus and An. dirus, with LC50 values of < 0.1, 2.22 and < 0.1%, respectively. Ten percent C. citratus gave the highest mortality rates (100%) 24 hours after application. This study demonstrates the potential for the essential oil of C. citratus to be used as an insecticide against 3 species of mosquitoes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / drug effects
  • Animals
  • Anopheles / drug effects
  • Culex / drug effects
  • Culicidae / drug effects*
  • Insect Vectors / drug effects*
  • Insecticides / chemistry
  • Insecticides / pharmacology*
  • Plant Oils / administration & dosage
  • Plant Oils / pharmacology*
  • Thailand


  • Insecticides
  • Plant Oils