Species identification of clinical Prevotella isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

J Clin Microbiol. 2012 Apr;50(4):1415-8. doi: 10.1128/JCM.06326-11. Epub 2012 Feb 1.

Abstract

The performance of matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) for species identification of Prevotella was evaluated and compared with 16S rRNA gene sequencing. Using a Bruker database, 62.7% of the 102 clinical isolates were identified to the species level and 73.5% to the genus level. Extension of the commercial database improved these figures to, respectively, 83.3% and 89.2%. MALDI-TOF MS identification of Prevotella is reliable but needs a more extensive database.

Publication types

  • Comparative Study

MeSH terms

  • Molecular Sequence Data
  • Prevotella / classification*
  • Prevotella / genetics
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Reference Standards
  • Sequence Analysis, RNA
  • Species Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / standards

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/JN867221
  • GENBANK/JN867322