Survival of rat functional dental pulp cells in vascularized tissue engineering chambers

Tissue Cell. 2012 Apr;44(2):111-21. doi: 10.1016/j.tice.2011.12.003. Epub 2012 Jan 31.


Regenerative endodontics aims to preserve, repair or regenerate the dental pulp tissue. Dental pulp stem cells, have a potential use in dental tissue generation. However, specific requirements to drive the dental tissue generation are still obscured. We established an in vivo model for studying the survival of dental pulp cells (DPC) and their potential to generate dental pulp tissue. DPC were mixed with collagen scaffold with or without slow release bone morphogenic protein 4 (BMP-4) and fibroblast growth factor 2 (FGF2). The cell suspension was transplanted into a vascularized tissue engineering chamber in the rat groin. Tissue constructs were harvested after 2, 4, 6, and 8 weeks and processed for histomorphological and immunohistochemical analysis. After 2 weeks newly formed tissue with new blood vessel formation were observed inside the chamber. DPC were found around dentin, particularly around the vascular pedicle and also close to the gelatin microspheres. Cell survival, was confirmed up to 8 weeks after transplantation. Dentin Sialophosphoprotein (DSPP) positive matrix production was detected in the chamber, indicating functionality of dental pulp progenitor cells. This study demonstrates the potential of our tissue engineering model to study rat dental pulp cells and their behavior in dental pulp regeneration, for future development of an alternative treatment using these techniques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Protein 4 / metabolism
  • Cell Survival
  • Collagen / metabolism
  • Dental Pulp / cytology*
  • Dental Pulp / metabolism
  • Dentin / blood supply
  • Dentin / metabolism
  • Dentin / physiology
  • Extracellular Matrix Proteins / metabolism
  • Fibroblast Growth Factor 2 / metabolism
  • Groin / blood supply
  • Groin / physiology
  • Humans
  • Immunohistochemistry
  • Male
  • Neovascularization, Physiologic*
  • Phosphoproteins / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Regeneration*
  • Sialoglycoproteins / metabolism
  • Stem Cell Transplantation
  • Stem Cells / metabolism
  • Stem Cells / physiology
  • Tissue Engineering / instrumentation*
  • Tissue Engineering / methods
  • Tissue Scaffolds


  • Bmp4 protein, rat
  • Bone Morphogenetic Protein 4
  • Extracellular Matrix Proteins
  • Phosphoproteins
  • Sialoglycoproteins
  • dentin sialophosphoprotein
  • Fibroblast Growth Factor 2
  • Collagen