Coriandrum Sativum L. Protects Human Keratinocytes From Oxidative Stress by Regulating Oxidative Defense Systems

Skin Pharmacol Physiol. 2012;25(2):93-9. doi: 10.1159/000335257. Epub 2012 Feb 1.

Abstract

Background: Oxidative radicals are major environmental causes of human skin damage. Oxidative defense factors, including nuclear factor erythroid-derived 2-related factor 2 (Nrf2), are centrally involved in repairing skin cells or protecting them from oxidative damage. Coriandrum sativum L. (coriander; CS) is a commonly consumed food and a traditional phytomedicine in Asia and Europe. In this study, we examined the protective effects of a standardized CS leaf extract against oxidative stress in human HaCaT keratinocytes.

Methods and results: CS significantly and dose-dependently protected cells against reduced cell viability caused by H2O2-induced damage, as assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Other assays demonstrated that CS protected HaCaT cells by increasing the levels of glutathione and activities of oxidative defense enzymes, such as superoxide dismutase and catalase. Moreover, it increased the expression of activated Nrf2, which plays a crucial role in protecting skin cells against oxidative stress.

Conclusion: These results suggest that CS protects human keratinocytes from H2O2-induced oxidative stress through antioxidant effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / metabolism
  • Catalase / metabolism
  • Cell Line
  • Cell Survival / drug effects
  • Coriandrum / chemistry*
  • Dose-Response Relationship, Drug
  • Glutathione / metabolism
  • Humans
  • Hydrogen Peroxide / toxicity
  • Keratinocytes / drug effects*
  • Keratinocytes / metabolism
  • Medicine, Traditional
  • NF-E2-Related Factor 2 / metabolism
  • Oxidative Stress / drug effects*
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Superoxide Dismutase / drug effects
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • NF-E2-Related Factor 2
  • Plant Extracts
  • Hydrogen Peroxide
  • Catalase
  • Superoxide Dismutase
  • Glutathione