Characterization of Nipah virus from outbreaks in Bangladesh, 2008-2010

Emerg Infect Dis. 2012 Feb;18(2):248-55. doi: 10.3201/eid1802.111492.


Nipah virus (NiV) is a highly pathogenic paramyxovirus that causes fatal encephalitis in humans. The initial outbreak of NiV infection occurred in Malaysia and Singapore in 1998-1999; relatively small, sporadic outbreaks among humans have occurred in Bangladesh since 2001. We characterized the complete genomic sequences of identical NiV isolates from 2 patients in 2008 and partial genomic sequences of throat swab samples from 3 patients in 2010, all from Bangladesh. All sequences from patients in Bangladesh comprised a distinct genetic group. However, the detection of 3 genetically distinct sequences from patients in the districts of Faridpur and Gopalganj indicated multiple co-circulating lineages in a localized region over a short time (January-March 2010). Sequence comparisons between the open reading frames of all available NiV genes led us to propose a standardized protocol for genotyping NiV; this protcol provides a simple and accurate way to classify current and future NiV sequences.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Bangladesh / epidemiology
  • Child
  • Conserved Sequence
  • Disease Outbreaks*
  • Female
  • Genetic Variation
  • Genome, Viral
  • Henipavirus Infections / epidemiology*
  • Henipavirus Infections / virology
  • Humans
  • Molecular Sequence Data
  • Molecular Typing
  • Nipah Virus / genetics*
  • Nipah Virus / isolation & purification
  • Phylogeny
  • Sequence Analysis, DNA
  • Seroepidemiologic Studies
  • Viral Proteins / chemistry
  • Viral Proteins / genetics


  • Viral Proteins