Abstract
Leonurine, an active alkaloid of Traditional Chinese Medicine Herba leonuri, displayed cardioprotective effects by anti-oxidative and anti-apoptotic activities in vitro and in vivo. Herein, we explored the effects and possible mechanisms of leonurine on lipopolysaccharide (LPS)-induced inflammatory responses in human umbilical vein endothelial cells (HUVEC). We found that leonurine pretreatment concentration-dependently attenuated LPS-induced mRNA expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and monocyte chemoattractant protein-1. Meanwhile, LPS-mediated expression/release of ICAM-1, VCAM-1, and cyclooxygenase-2, and tumor necrosis factor-α was also reduced by leonurine. In addition, we confirmed that leonurine suppressed degradation of IκBα and phosphorylation of nuclear factor-κB (NF-κB) p65 as well as production of intracellular reactive oxygen species in a concentration dependent manner. Furthermore, the cytoprotective enzyme heme oxygenase-1 could be upregulated in leonurine-treated HUVEC. Our present results indicated leonurine exerted beneficial effects in inflammatory conditions partly through inhibition of reactive oxygen species and NF-κB signaling pathways.
Copyright © 2012. Published by Elsevier B.V.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents / pharmacology*
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Cells, Cultured
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Chemokine CCL2 / genetics
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Chemokine CCL2 / metabolism
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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E-Selectin / genetics
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E-Selectin / metabolism
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism
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Gallic Acid / analogs & derivatives*
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Gallic Acid / pharmacology
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Heme Oxygenase-1 / genetics
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Heme Oxygenase-1 / metabolism
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Human Umbilical Vein Endothelial Cells / drug effects*
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Human Umbilical Vein Endothelial Cells / metabolism
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Humans
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I-kappa B Proteins / genetics
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I-kappa B Proteins / metabolism
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Inflammation / chemically induced
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Inflammation / drug therapy
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Inflammation / metabolism
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Intercellular Adhesion Molecule-1 / genetics
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Intercellular Adhesion Molecule-1 / metabolism
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Lipopolysaccharides / pharmacology*
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NF-KappaB Inhibitor alpha
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NF-kappa B / genetics
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NF-kappa B / metabolism*
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Phosphorylation / drug effects
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Phosphorylation / genetics
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RNA, Messenger / genetics
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Reactive Oxygen Species / antagonists & inhibitors
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Reactive Oxygen Species / metabolism*
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Signal Transduction / drug effects
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Signal Transduction / genetics
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
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Vascular Cell Adhesion Molecule-1 / genetics
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Vascular Cell Adhesion Molecule-1 / metabolism
Substances
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Anti-Inflammatory Agents
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Chemokine CCL2
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E-Selectin
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I-kappa B Proteins
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Lipopolysaccharides
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NF-kappa B
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NFKBIA protein, human
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RNA, Messenger
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Reactive Oxygen Species
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1
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leonurine
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Intercellular Adhesion Molecule-1
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NF-KappaB Inhibitor alpha
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Gallic Acid
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Heme Oxygenase-1
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Cyclooxygenase 2
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PTGS2 protein, human