Three-dimensional organization of block copolymers on "DNA-minimal" scaffolds

J Am Chem Soc. 2012 Mar 7;134(9):4280-6. doi: 10.1021/ja210313p. Epub 2012 Feb 22.


Here, we introduce a 3D-DNA construction method that assembles a minimum number of DNA strands in quantitative yield, to give a scaffold with a large number of single-stranded arms. This DNA frame is used as a core structure to organize other functional materials in 3D as the shell. We use the ring-opening metathesis polymerization (ROMP) to generate block copolymers that are covalently attached to DNA strands. Site-specific hybridization of these DNA-polymer chains on the single-stranded arms of the 3D-DNA scaffold gives efficient access to DNA-block copolymer cages. These biohybrid cages possess polymer chains that are programmably positioned in three dimensions on a DNA core and display increased nuclease resistance as compared to unfunctionalized DNA cages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Models, Molecular
  • Molecular Structure
  • Particle Size
  • Polymers / chemistry*
  • Surface Properties


  • Polymers
  • DNA