Concanavalin A immobilized poly(ethylene glycol dimethacrylate) based affinity cryogel matrix and usability of invertase immobilization

J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Mar 1;887-888:73-8. doi: 10.1016/j.jchromb.2012.01.014. Epub 2012 Jan 24.

Abstract

Concanavalin A (Con A) immobilized supermacroporous poly(ethylene glycol dimethacrylate) [poly(EGDMA)] monolithic cryogel column was prepared by radical cryocopolymerization of EGDMA as a monomer and N,N'-methylene-bisacrylamide as a crosslinker. Bioligand Con A was then immobilized by covalent binding onto poly(EGDMA) cryogel via glutaraldehyde activation [Con A-poly(EGDMA)]. Con A-poly(EGDMA) cryogel was characterized by swelling studies and scanning electron microscopy. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10-50 μm size. The equilibrium swelling degree of the cryogel was 15.01 g H₂O/g dry cryogel. Con A-poly(EGDMA) cryogel was used in the adsorption/desorption of invertase from aqueous solutions. The maximum amount of invertase adsorption from aqueous solution in acetate buffer was 55.45 mg/g polymer at pH 5.0. Con A-poly(EGDMA) cryogels were used for repetitive adsorption/desorption of invertase without noticeable loss in invertase adsorption capacity after 10 cycles.

MeSH terms

  • Adsorption
  • Chromatography, Affinity / instrumentation*
  • Chromatography, Affinity / methods
  • Concanavalin A / chemistry*
  • Concanavalin A / metabolism
  • Cryogels / chemistry*
  • Enzymes, Immobilized / chemistry*
  • Enzymes, Immobilized / metabolism
  • Hydrogen-Ion Concentration
  • Methacrylates / chemistry*
  • Osmolar Concentration
  • Polyethylene Glycols / chemistry*
  • Porosity
  • beta-Fructofuranosidase / chemistry*
  • beta-Fructofuranosidase / isolation & purification
  • beta-Fructofuranosidase / metabolism

Substances

  • Cryogels
  • Enzymes, Immobilized
  • Methacrylates
  • poly(ethylene glycol)-dimethacrylate
  • Concanavalin A
  • Polyethylene Glycols
  • beta-Fructofuranosidase