Vitamin D 25-hydroxylase - Four decades of searching, are we there yet?

Arch Biochem Biophys. 2012 Jul 1;523(1):30-6. doi: 10.1016/ Epub 2012 Jan 31.


Bioactivation of vitamin D(3) involves 25-hydroxylation and subsequent 1α-hydroxylation to produce 1α,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)], the active hormone. Six cytochrome P450 enzymes (CYP27A1, CYP2R1, CYP2J2/3, CYP3A4, CYP2D25 and CYP2C11) catalyzing the initial 25-hydroxylation step are reviewed, and their physiological relevance as vitamin D 25-hydroxylases in vivo is discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cholecalciferol / metabolism*
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / metabolism*
  • Humans
  • Intracellular Space / enzymology
  • Protein Transport


  • Cholecalciferol
  • Cytochrome P-450 Enzyme System