Metabolism of hyperpolarized [1-¹³C]pyruvate in the isolated perfused rat lung - an ischemia study

NMR Biomed. 2012 Oct;25(10):1113-8. doi: 10.1002/nbm.2777. Epub 2012 Feb 7.


We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p < 0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Carbon Isotopes
  • In Vitro Techniques
  • Ischemia / metabolism*
  • Isotope Labeling
  • Lactic Acid / metabolism
  • Lung / metabolism*
  • Magnetic Resonance Spectroscopy*
  • Male
  • Perfusion / methods*
  • Principal Component Analysis
  • Pyruvic Acid / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion


  • Carbon Isotopes
  • Lactic Acid
  • Pyruvic Acid