SARS CoV subunit vaccine: antibody-mediated neutralisation and enhancement

Hong Kong Med J. 2012 Feb;18 Suppl 2:31-6.

Abstract

1. A SARS vaccine was produced based on recombinant native full-length Spike-protein trimers (triSpike) and efficient establishment of a vaccination procedure in rodents. 2. Antibody-mediated enhancement of SARS-CoV infection with anti-SARS-CoV Spike immune-serum was observed in vitro. 3. Antibody-mediated infection of SARS-CoV triggers entry into human haematopoietic cells via an FcγR-dependent and ACE2-, pH-, cysteine-protease-independent pathways. 4. The antibody-mediated enhancement phenomenon is not a mandatory component of the humoral immune response elicited by SARS vaccines, as pure neutralising antibody only could be obtained. 5. Occurrence of immune-mediated enhancement of SARS-CoV infection raises safety concerns regarding the use of SARS-CoV vaccine in humans and enables new ways to investigate SARS pathogenesis (tropism and immune response deregulation).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin-Converting Enzyme 2
  • Animals
  • Antibodies, Neutralizing / metabolism*
  • Antibodies, Viral / metabolism
  • Antibody-Dependent Enhancement*
  • Cell Line, Tumor
  • Cysteine Proteases / metabolism
  • Humans
  • Hydrogen-Ion Concentration
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Monocytes
  • Peptidyl-Dipeptidase A / metabolism
  • Receptors, Fc / metabolism
  • SARS Virus / immunology*
  • SARS Virus / physiology
  • Severe Acute Respiratory Syndrome / immunology*
  • Spike Glycoprotein, Coronavirus
  • Vaccines
  • Viral Envelope Proteins / immunology*
  • Virus Internalization*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Membrane Glycoproteins
  • Receptors, Fc
  • Spike Glycoprotein, Coronavirus
  • Vaccines
  • Viral Envelope Proteins
  • Cysteine Proteases
  • Peptidyl-Dipeptidase A
  • ACE2 protein, human
  • Ace2 protein, mouse
  • Angiotensin-Converting Enzyme 2