A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin α as fusion tag

Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7.


Enhanced green fluorescent protein (EGFP) and its yellow variant (Venus) are weakly dimeric under physiological conditions. We designed a simple method to evaluate the dimeric tendency of fluorescent proteins in living mammalian cells. A novel single mutation, A206L, interfering with the hydrophobic interactions of the dimer interface in Venus, contributed to its monomerization, and was as effective as the A206K mutation in this assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells / chemistry
  • Cytological Techniques / methods*
  • Green Fluorescent Proteins
  • Hydrophobic and Hydrophilic Interactions
  • Luminescent Proteins*
  • Methods
  • Mutation, Missense
  • Protein Multimerization*
  • Recombinant Fusion Proteins
  • alpha Karyopherins* / genetics


  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • alpha Karyopherins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins