Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2919-24. doi: 10.1073/pnas.1111662109. Epub 2012 Feb 6.

Abstract

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cerebral Cortex / cytology
  • Imaging, Three-Dimensional / methods*
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Neurons / physiology*
  • Reproducibility of Results