Early cystic fibrosis lung disease detected by bronchoalveolar lavage and lung clearance index

Am J Respir Crit Care Med. 2012 Apr 15;185(8):862-73. doi: 10.1164/rccm.201109-1631OC. Epub 2012 Feb 9.

Abstract

Rationale: Unrecognized airway infection and inflammation in young children with cystic fibrosis (CF) may lead to irreversible lung disease; therefore early detection and treatment is highly desirable.

Objectives: To determine whether the lung clearance index (LCI) is a sensitive and repeatable noninvasive measure of airway infection and inflammation in newborn-screened children with CF.

Methods: Forty-seven well children with CF (mean age, 1.55 yr) and 25 healthy children (mean age, 1.26 yr) underwent multiple-breath washout testing. LCI within and between-test variability was assessed. Children with CF also had surveillance bronchoalveolar lavage performed.

Measurements and main results: The mean (SD) LCI in healthy children was 6.45 (0.49). The LCI was higher in children with CF (7.21 [0.81]; P < 0.001). The upper limit of normal for the LCI was 7.41. Fifteen (32%) children with CF had an elevated LCI. LCI measurements were repeatable and reproducible. Airway infection was present in 17 (36%) children with CF, including 7 (15%) with Pseudomonas aeruginosa. Polymicrobial growth was associated with worse inflammation. The LCI was higher in children with Pseudomonas (7.92 [1.16]) than in children without Pseudomonas (7.02 [0.56]) (P = 0.038). The LCI correlated with bronchoalveolar lavage IL-8 (R(2) = 0.20, P = 0.004) and neutrophil count (R(2) = 0.21, P = 0.001). An LCI below the upper limit of normality had a high negative predictive value (93%) in excluding Pseudomonas.

Conclusions: The LCI is elevated early in CF, especially in the presence of Pseudomonas and airway inflammation. The LCI is a feasible, repeatable, and sensitive noninvasive marker of lung disease in young children with CF.

Publication types

  • Comparative Study

MeSH terms

  • Australia
  • Bronchoalveolar Lavage Fluid / microbiology*
  • Case-Control Studies
  • Child, Preschool
  • Cystic Fibrosis / complications
  • Cystic Fibrosis / diagnosis*
  • Early Diagnosis
  • Female
  • Forced Expiratory Volume
  • Humans
  • Infant
  • Lung Diseases / diagnosis*
  • Lung Diseases / etiology
  • Lung Diseases / microbiology
  • Male
  • Pseudomonas Infections / diagnosis
  • Pseudomonas Infections / etiology
  • Pseudomonas aeruginosa / isolation & purification
  • Pulmonary Gas Exchange / physiology*
  • ROC Curve
  • Reference Values
  • Retrospective Studies
  • Severity of Illness Index
  • Statistics, Nonparametric