In vitro and in vivo efficacy of aqueous extract of Caryocar brasiliense Camb. to control gastrointestinal nematodes in sheep

Parasitol Res. 2012 Jul;111(1):325-30. doi: 10.1007/s00436-012-2843-8. Epub 2012 Feb 11.


A major problem faced in sheep rearing has been the rapid acquisition of anthelminthic-resistant populations of gastrointestinal nematodes. In the search for alternatives, aqueous extract of the peel of Caryocar brasiliense was evaluated for larval development inhibition, egg-hatching inhibition, and fecal nematode egg count reduction in sheep. For in vivo analysis, the doses were calculated according to a 10% lethal dose derived from acute toxicity tests in mice, and the efficacy was evaluated for two periods following oral administration of the extract. Egg-hatching inhibition at concentrations of 15 and 7.5 mg/ml was significantly higher than observed in negative controls with distilled water. For larval development inhibition, all concentrations showed anthelminthic activity significantly higher than controls and were not significantly different from ivermectin treatment. The LC(90) of larval development inhibition was 53.19 mg/ml. In vivo analysis for first and second weeks after treatment found 32.2% and 33% anthelminthic efficacy, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthelmintics / administration & dosage
  • Anthelmintics / adverse effects
  • Anthelmintics / isolation & purification
  • Anthelmintics / pharmacology*
  • Ericales / chemistry*
  • Feces / parasitology
  • Helminthiasis / parasitology
  • Helminthiasis, Animal / parasitology*
  • Intestinal Diseases / parasitology
  • Intestinal Diseases / veterinary*
  • Intestinal Diseases, Parasitic
  • Mice
  • Nematoda / drug effects*
  • Nematode Infections / parasitology*
  • Parasite Egg Count
  • Plant Extracts / administration & dosage
  • Plant Extracts / adverse effects
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Sheep
  • Survival Analysis


  • Anthelmintics
  • Plant Extracts

Supplementary concepts

  • Intestinal helminthiasis