Spatiotemporal control of gene expression by a light-switchable transgene system

Nat Methods. 2012 Feb 12;9(3):266-9. doi: 10.1038/nmeth.1892.


We developed a light-switchable transgene system based on a synthetic, genetically encoded light-switchable transactivator. The transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. This transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / genetics*
  • DNA / radiation effects*
  • Gene Expression / genetics*
  • Gene Expression / radiation effects*
  • Light
  • Radiation Dosage
  • Transgenes / genetics*
  • Transgenes / radiation effects*


  • DNA