Accuracy of correction in modal sensorless adaptive optics

Opt Express. 2012 Jan 30;20(3):2598-612. doi: 10.1364/OE.20.002598.

Abstract

We investigate theoretically and experimentally the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. On the example of two-photon fluorescence imaging, we show that using a suitable number of measurements, precise correction can be obtained for up to 2 radians rms aberrations without optimising the aberration modes used for correction. We also investigate the number of photons required for accurate correction when signal acquisition is shot-noise limited. We show that only 10(4) to 10(5) photons are required for complete correction so that the correction process can be implemented with limited extra-illumination and associated photoperturbation. Finally, we provide guidelines for implementing an optimal correction algorithm depending on the experimental conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Artifacts*
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods*
  • Lenses*
  • Microscopy / instrumentation*
  • Reproduction
  • Sensitivity and Specificity
  • Transducers