Microwave electromagnetic field regulates gene expression in T-lymphoblastoid leukemia CCRF-CEM cell line exposed to 900 MHz

Electromagn Biol Med. 2012 Mar;31(1):1-18. doi: 10.3109/15368378.2011.596251.


Electric, magnetic, and electromagnetic fields are ubiquitous in our society, and concerns have been expressed regarding possible adverse effects of these exposures. Research on Extremely Low-Frequency (ELF) magnetic fields has been performed for more than two decades, and the methodology and quality of studies have improved over time. Studies have consistently shown increased risk for childhood leukemia associated with ELF magnetic fields. There are still inadequate data for other outcomes. More recently, focus has shifted toward Radio Frequencies (RF) exposures from mobile telephony. There are no persuasive data suggesting a health risk, but this research field is still immature with regard to the quantity and quality of available data. This technology is constantly changing and there is a need for continued research on this issue. To investigate whether exposure to high-frequency electromagnetic fields (EMF) could induce adverse health effects, we cultured acute T-lymphoblastoid leukemia cells (CCRF-CEM) in the presence of 900 MHz MW-EMF generated by a transverse electromagnetic (TEM) cell at short and long exposure times. We evaluated the effect of high-frequency EMF on gene expression and we identified functional pathways influenced by 900 MHz MW-EMF exposure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics
  • Apoptosis / radiation effects
  • Cell Cycle Checkpoints / genetics
  • Cell Cycle Checkpoints / radiation effects
  • Cell Line, Tumor
  • Cell Survival / genetics
  • Cell Survival / radiation effects
  • Chromosomes / genetics
  • Chromosomes / radiation effects
  • DNA Damage / genetics
  • DNA Repair / genetics
  • DNA Repair / radiation effects
  • Electromagnetic Fields / adverse effects*
  • Humans
  • Microwaves / adverse effects*
  • Neovascularization, Physiologic / genetics
  • Neovascularization, Physiologic / radiation effects
  • Oligonucleotide Array Sequence Analysis
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / pathology*
  • Time Factors
  • Transcriptome / radiation effects*