Light-emitting diodes at 830 and 850 nm inhibit melanin synthesis in vitro

Acta Derm Venereol. 2012 Nov;92(6):675-80. doi: 10.2340/00015555-1319.

Abstract

Treatment of hyperpigmentation remains a challenge. Because of the positive effects of low-energy Nd:YAG lasers on the treatment of melasma, it is suggested that laser-like light-emitting diodes (LEDs) can potentially ameliorate hyperpigmentation. We evaluated the effect of seven different LED wavelengths on melanogenesis. LED irradiation at 830 nm (dose-dependent, from 1 to 20 J/cm2) and 850 nm (1 J/cm2) significantly reduced melanin production and tyrosinase expression, not only in a normal human melanocyte monoculture both with and without forskolin stimulation but also in a three-dimensional multiple cell type culture. It reduced melanin content via inactivation of the apoptosis signal-regulating kinase and extracellular signal-regulated kinase 1/2 pathways. The level of phosphorylated cyclic AMP response element-binding protein was also decreased by LED irradiation. Moreover, LED irradiation reduced melanogenesis through decreased expression of tyrosinase family genes (tyrosinase-related protein-1 and 2, and microphthalmia-associated transcription factor). These results indicate that LEDs could potentially be used to treat melanin-overproducing skin conditions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Colforsin / pharmacology
  • Cyclic AMP / metabolism
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • Dose-Response Relationship, Radiation
  • Enzyme Activation
  • Humans
  • Intramolecular Oxidoreductases / metabolism
  • Lasers, Semiconductor*
  • MAP Kinase Kinase Kinases / metabolism
  • Melanins / biosynthesis*
  • Melanocytes / drug effects
  • Melanocytes / metabolism
  • Melanocytes / radiation effects*
  • Membrane Glycoproteins / metabolism
  • Microphthalmia-Associated Transcription Factor / metabolism
  • Mitogen-Activated Protein Kinase 1 / metabolism
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Monophenol Monooxygenase / metabolism
  • Oxidoreductases / metabolism
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction / radiation effects
  • Skin Pigmentation / drug effects
  • Skin Pigmentation / radiation effects*

Substances

  • CREB1 protein, human
  • Cyclic AMP Response Element-Binding Protein
  • MITF protein, human
  • Melanins
  • Membrane Glycoproteins
  • Microphthalmia-Associated Transcription Factor
  • Colforsin
  • Cyclic AMP
  • Oxidoreductases
  • TYRP1 protein, human
  • Monophenol Monooxygenase
  • Proto-Oncogene Proteins c-akt
  • MAPK1 protein, human
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • MAP Kinase Kinase Kinases
  • Intramolecular Oxidoreductases
  • dopachrome isomerase