Discovery of ALK-PTPN3 Gene Fusion From Human Non-Small Cell Lung Carcinoma Cell Line Using Next Generation RNA Sequencing

Genes Chromosomes Cancer. 2012 Jun;51(6):590-7. doi: 10.1002/gcc.21945. Epub 2012 Feb 15.

Abstract

An increasing number of chromosomal aberrations is being identified in solid tumors providing novel biomarkers for various types of cancer and new insights into the mechanisms of carcinogenesis. We applied next generation sequencing technique to analyze the transcriptome of the non-small cell lung carcinoma (NSCLC) cell line H2228 and discovered a fusion transcript composed of multiple exons of ALK (anaplastic lymphoma receptor tyrosine kinase) and PTPN3 (protein tyrosine phosphatase, nonreceptor Type 3). Detailed analysis of the genomic structure revealed that a portion of genomic region encompassing Exons 10 and 11 of ALK has been translocated into the intronic region between Exons 2 and 3 of PTPN3. The key net result appears to be the null mutation of one allele of PTPN3, a gene with tumor suppressor activity. Consistently, ectopic expression of PTPN3 in NSCLC cell lines led to inhibition of colony formation. Our study confirms the utility of next generation sequencing as a tool for the discovery of somatic mutations and has led to the identification of a novel mutation in NSCLC that may be of diagnostic, prognostic, and therapeutic importance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaplastic Lymphoma Kinase
  • Base Sequence
  • Carcinoma, Non-Small-Cell Lung / diagnosis
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Cell Line, Tumor
  • Humans
  • Lung Neoplasms / diagnosis
  • Lung Neoplasms / genetics*
  • Molecular Sequence Data
  • Oncogene Proteins, Fusion / genetics*
  • Protein Tyrosine Phosphatase, Non-Receptor Type 3 / genetics*
  • RNA, Neoplasm / chemistry
  • RNA, Neoplasm / genetics*
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Sequence Analysis, DNA

Substances

  • Oncogene Proteins, Fusion
  • RNA, Neoplasm
  • ALK protein, human
  • Anaplastic Lymphoma Kinase
  • Receptor Protein-Tyrosine Kinases
  • PTPN3 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 3