Characterization of influenza A virus with nine segments: effect gene segment on virus property

Res Vet Sci. 2012 Oct;93(2):1076-80. doi: 10.1016/j.rvsc.2011.12.020. Epub 2012 Feb 14.

Abstract

The influenza A virus genome consists of eight segments of negative-strand RNA. In previous study, we generated a recombinant influenza virus with nine segments by reverse genetics. In present study, we evaluated characteristics of the recombinant influenza virus. The recombinant virus exhibited similar property to wild-type virus on virion morphology, virion composition, plaque phenotype and other aspects. Whereas, the recombinant virus propagated to lower titers than did wild-type virus in cells and mice, and there was decreased protein level and vRNA incorporation in the recombinant virions compared to wild-type H9N2 virions. Our results indicated that influenza A virus with eight segments exhibits more advantages than the virus with nine segments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • Dogs
  • Electrophoresis, Polyacrylamide Gel
  • Gene Deletion
  • Gene Expression Regulation, Viral / physiology
  • Influenza A Virus, H9N2 Subtype / genetics*
  • Kinetics
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron
  • Mutagenesis, Insertional
  • Orthomyxoviridae Infections / virology*
  • RNA, Viral / genetics
  • Virion

Substances

  • RNA, Viral