Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy screening

Fertil Steril. 2012 Apr;97(4):819-24. doi: 10.1016/j.fertnstert.2012.01.115. Epub 2012 Feb 18.


Objective: To develop and validate a quantitative real-time polymerase chain reaction (qPCR)-based method for blastocyst trophectoderm comprehensive chromosome screening (CCS) of aneuploidy.

Design: Prospective, randomized, and blinded.

Setting: Academic center for reproductive medicine.

Patient(s): Nine cell lines were obtained from a commercial cell line repository, and 71 discarded human blastocysts were obtained from 24 IVF patients that underwent preimplantation genetic screening.

Intervention(s): None.

Main outcome measure(s): Consistency of qPCR diagnosis of aneuploidy compared with either conventional karyotyping of cell lines or microarray-based diagnoses of human blastocysts.

Result(s): Samples from nine cell lines with well characterized karyotypes were diagnosed by qPCR with 97.6% (41/42) consistency. After applying a minimum threshold for concurrence, 100% consistency was achieved. Developmentally normal blastocysts designated as aneuploid or arrested blastocysts designated as euploid by single-nucleotide polymorphism microarray analyses were assigned identical 24 chromosome diagnoses by qPCR in 98.6% of cases (70/71). Overall euploidy (n = 37) and aneuploidy (n = 34) were assigned with 100% consistency. Data was obtained for both sample types in 4 hours.

Conclusion(s): These data demonstrate the first qPCR technology capable of accurate aneuploidy screening of all 24 chromosomes in 4 hours. This methodology provides an opportunity to evaluate trophectoderm biopsies with subsequent fresh euploid blastocyst transfer. Randomized controlled trials to investigate the clinical efficacy of qPCR-based CCS are currently underway.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Academic Medical Centers
  • Aneuploidy*
  • Blastocyst / pathology*
  • Cell Line
  • Female
  • Fertilization in Vitro / adverse effects*
  • Genetic Testing*
  • Humans
  • Karyotyping
  • New Jersey
  • Oligonucleotide Array Sequence Analysis
  • Predictive Value of Tests
  • Pregnancy
  • Pregnancy Complications / diagnosis*
  • Pregnancy Complications / genetics
  • Preimplantation Diagnosis / methods*
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction*
  • Reproducibility of Results