A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination

Neurosci Res. 2012 May;73(1):85-91. doi: 10.1016/j.neures.2012.02.003. Epub 2012 Feb 17.


Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Nucleotidyltransferases / physiology*
  • Gene Knock-In Techniques
  • Genes, Reporter / physiology*
  • Green Fluorescent Proteins / physiology*
  • Integrases / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Proteins / physiology*
  • RNA, Untranslated
  • Recombination, Genetic / physiology*


  • Gt(ROSA)26Sor non-coding RNA, mouse
  • Proteins
  • RNA, Untranslated
  • Green Fluorescent Proteins
  • Cre recombinase
  • DNA Nucleotidyltransferases
  • FLP recombinase
  • Integrases