Specific DNA duplex formation at an artificial lipid bilayer: towards a new DNA biosensor technology

Chem Biodivers. 2012 Feb;9(2):272-81. doi: 10.1002/cbdv.201100298.


A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayer-H(2) O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface.

MeSH terms

  • Biosensing Techniques*
  • DNA / chemistry*
  • DNA Replication
  • Humans
  • Lipid Bilayers / chemistry*
  • Lipids
  • Oligonucleotides / chemistry*
  • Spectrometry, Fluorescence
  • Water / chemistry*


  • Lipid Bilayers
  • Lipids
  • Oligonucleotides
  • Water
  • DNA