Europium-labeled activity-based probe through click chemistry: absolute serine protease quantification using (153)Eu isotope dilution ICP/MS

Angew Chem Int Ed Engl. 2012 Apr 2;51(14):3358-63. doi: 10.1002/anie.201108277. Epub 2012 Feb 17.

Abstract

Click and analyze: the titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153)Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153)Eu(NO(3))(3) isotopic standard.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Chromatography, High Pressure Liquid
  • Click Chemistry*
  • Europium / chemistry*
  • Heterocyclic Compounds, 1-Ring / chemistry
  • Isotope Labeling
  • Mass Spectrometry*
  • Serine Proteases / analysis*
  • Sulfinic Acids / chemistry

Substances

  • Heterocyclic Compounds, 1-Ring
  • Sulfinic Acids
  • 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid
  • Europium
  • sulfuryl fluoride
  • Serine Proteases