Genotype probabilities at intermediate generations in the construction of recombinant inbred lines

Genetics. 2012 Feb;190(2):403-12. doi: 10.1534/genetics.111.132647.

Abstract

The mouse Collaborative Cross (CC) is a panel of eight-way recombinant inbred lines: eight diverse parental strains are intermated, followed by repeated sibling mating, many times in parallel, to create a new set of inbred lines whose genomes are random mosaics of the genomes of the original eight strains. Many generations are required to reach inbreeding, and so a number of investigators have sought to make use of phenotype and genotype data on mice from intermediate generations during the formation of the CC lines (so-called pre-CC mice). The development of a hidden Markov model for genotype reconstruction in such pre-CC mice, on the basis of incompletely informative genetic markers (such as single-nucleotide polymorphisms), formally requires the two-locus genotype probabilities at an arbitrary generation along the path to inbreeding. In this article, I describe my efforts to calculate such probabilities. While closed-form solutions for the two-locus genotype probabilities could not be derived, I provide a prescription for calculating such probabilities numerically. In addition, I present a number of useful quantities, including single-locus genotype probabilities, two-locus haplotype probabilities, and the fixation probability and map expansion at each generation along the course to inbreeding.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Crosses, Genetic*
  • Genetic Loci
  • Genotype*
  • Inbreeding
  • Mice
  • Mice, Inbred Strains / genetics*
  • Models, Theoretical
  • Probability
  • X Chromosome