3D elemental sensitive imaging using transmission X-ray microscopy

Anal Bioanal Chem. 2012 Sep;404(5):1297-301. doi: 10.1007/s00216-012-5818-9. Epub 2012 Feb 19.


Determination of the heterogeneous distribution of metals in alloy/battery/catalyst and biological materials is critical to fully characterize and/or evaluate the functionality of the materials. Using synchrotron-based transmission x-ray microscopy (TXM), it is now feasible to perform nanoscale-resolution imaging over a wide X-ray energy range covering the absorption edges of many elements; combining elemental sensitive imaging with determination of sample morphology. We present an efficient and reliable methodology to perform 3D elemental sensitive imaging with excellent sample penetration (tens of microns) using hard X-ray TXM. A sample of an Al-Si piston alloy is used to demonstrate the capability of the proposed method.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Alloys / chemistry*
  • Aluminum / analysis*
  • Equipment Design
  • Imaging, Three-Dimensional / methods*
  • Microscopy / instrumentation
  • Microscopy / methods*
  • Silicon / analysis*
  • Synchrotrons
  • X-Rays


  • Alloys
  • Aluminum
  • Silicon