Anti-inflammatory and anti-apoptotic effects of Crataegus oxyacantha on isoproterenol-induced myocardial damage

Mol Cell Biochem. 2012 Aug;367(1-2):1-8. doi: 10.1007/s11010-012-1251-9. Epub 2012 Feb 15.


This study was designed to evaluate the anti-inflammatory and anti-apoptotic effects of the alcoholic extract of the berries of Crataegus oxyacantha (AEC), a medicinal herb, on isoproterenol-induced myocardial infarction (MI) in a rat model. Three groups of Wistar albino rats, each comprising six animals, were selected for this study. Group I rats served as control. Group II rats were given isoproterenol (85 mg/kg body weight) subcutaneously on 59th and 60th days. Group III rats were given AEC (0.5 ml/100 g body weight/day), orally on a daily basis for 60 days, and isoproterenol (85 mg/kg body weight, subcutaneously) was given on 59th and 60th days. On the 61st day, the animals were sacrificed, and marker enzymes like lactate dehydrogenase (LDH) and creatine kinase (CK) were estimated in serum. In the heart tissue sample, antioxidant status, lipid peroxidation and anti-inflammatory properties of AEC were determined. Isoproterenol significantly increased the release of LDH, CK in serum, decreased the antioxidant status in the heart along with an increase in lipid peroxidation. Nitritive stress and apoptosis were seen in isoproterenol-induced rat heart. Pre-treatment with the AEC for 60 days had a significant effect on all the above factors and maintained near normal status. The study confirms the protective effect of AEC against isoproterenol-induced inflammation and apoptosis-associated MI in rats.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / therapeutic use
  • Apoptosis / drug effects*
  • Catalase / metabolism
  • Crataegus
  • Creatine Kinase / blood
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism
  • Cytochromes c / metabolism
  • DNA Fragmentation
  • Drug Evaluation, Preclinical
  • Fruit / chemistry
  • Isoproterenol
  • L-Lactate Dehydrogenase / blood
  • Lipid Peroxidation
  • Male
  • Mitochondria, Heart / metabolism
  • Myocardial Infarction / blood
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / drug therapy*
  • Myocardium / enzymology
  • Myocardium / pathology
  • Nitric Oxide Synthase Type II / metabolism
  • Nitrites / metabolism
  • Phytotherapy
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism


  • Anti-Inflammatory Agents
  • Nitrites
  • Plant Extracts
  • crataegus extract
  • Cytochromes c
  • L-Lactate Dehydrogenase
  • Catalase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat
  • Cyclooxygenase 2
  • Ptgs2 protein, rat
  • Superoxide Dismutase
  • Creatine Kinase
  • Isoproterenol